Protective clothing is generally worn by any beekeeper whilst collecting honey and caring for the beehives. Even though several layers are worn, a bee can sting through jeans and clothing quite easily! If the cloth is tight against your skin, a bee can sting you through a layer of clothing.
You cannot start a bee colony with a queen bee alone. A bee colony is a community of bees, where different bees have different functions, and they all depend on one another to fulfill their part for the colony to survive. The queen is pretty much helpless on her own and will not survive on her own, nor be able to build a colony without other bees.